Anti-SHMT2 Antibody Picoband®

SKU: A03233-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,IF,ICC/IF,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunofluorescence, 5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: SHMT2

Specificity:

Background: This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human SHMT2 recombinant protein (Position: A9-H504). Human SHMT2 shares 95.1% amino acid (aa) sequence identity with mouse SHMT2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bogenhagen, D. F., Rousseau, D., Burke, S. The layered structure of human mitochondrial DNA nucleoids. J. Biol. Chem. 283: 3665-3675, 2008. 2. Cao, J., Sun, L., Aramsangtienchai, P., Spiegelman, N. A., Zhang, X., Huang, W., Seto, E., Lin, H. HDAC11 regulates type I interferon signaling through defatty-acylation of SHMT2. Proc. Nat. Acad. Sci. 116: 5487-5492, 2019. 3. Garcia-Cazorla, A., Verdura, E., Julia-Palacios, N., Anderson, E. N., Goicoechea, L., Planas-Serra, L., Tsogtbaatar, E., Dsouza, N. R., Schluter, A., Urreizti, R. Tarnowski, J. M., Gavrilova, R. H., and 15 others. Impairment of the mitochondrial one-carbon metabolism enzyme SHMT2 causes a novel brain and heart developmental syndrome. Acta Neuropath. 140: 971-975, 2020.

Uniprot ID: P34897

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 56 kDa

Calculated Molecular Weight: 56 kDa

Gene ID: 6472

Protein Name: Serine hydroxymethyltransferase, mitochondrial

Gene Full Name: serine hydroxymethyltransferase 2

Synonyms: GLYA; Serine methylase; SHMT; SHMT2