Anti-SDHA Antibody Picoband®

SKU: PA1717

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: SDHA

Specificity: No cross reactivity with other proteins.

Background: SDHA (Succinate dehydrogenase complex, subunit a, flavoprotein), also called SDH1, HOMOLOG OF, is a protein that in humans is encoded by the SDHA gene. The SDHA gene is mapped on 5p15.33. The SDHA gene is highly polymorphic. The gene that codes for the SDHA protein is nuclear, even though the protein is located in the inner membrane of the mitochondria. Heterozygous carriers of an SDHA mutation do not developparagangliomas as has been seen for mutations in the other subunits. In vitro functional expression studies in the yeast homolog showed that the mutation resulted in a loss of SDH activity and rendered the mutant SDHA protein more susceptible to proteolysis. The findings indicated that SDHA, like other SDH subunits, can act as a tumor suppressor gene.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SDHA, identical to the related rat and mouse sequences.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. DiMauro, S., Schon, E. A. Nuclear power and mitochondrial disease. Nature Genet. 19: 214-215, 1998. 2. Hirawake, H., Wang, H., Kuramochi, T., Kojima, S., Kita, K. Human complex II (succinate-ubiquinone oxidoreductase): cDNA cloning of the flavoprotein (Fp) subunit of liver mitochondria. J. Biochem. 116: 221-227, 1994. 3. Morris, A. A. M., Farnsworth, L., Ackrell, B. A. C., Turnbull, D. M., Birch-Machin, M. A. The cDNA sequence of the flavoprotein subunit of human heart succinate dehydrogenase. Biochim. Biophys. Acta 1185: 125-128, 1994.

Uniprot ID: P31040

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 72 kDa

Calculated Molecular Weight: 72692 MW

Gene ID: 6389

Protein Name: Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Gene Full Name: succinate dehydrogenase complex flavoprotein subunit A

Synonyms: FP; SDH2; SDHA; SDHF