Anti-S100A4 Antibody Picoband®

SKU: A01217-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: S100A4

Specificity:

Background: S100 calcium-binding protein A4 (S100A4) is a protein that in humans is encoded by the S100A4 gene. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Immunogen: E.coli-derived human S100A4 recombinant protein (Position: A2-K101).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Ambartsumian, N., Tarabykina, S., Grigorian, M., Tulchinsky, E., Hulgaard, E., Georgiev, G., Lukanidin, E. Characterization of two splice variants of metastasis-associated human mts1 gene. Gene 159: 125-130, 1995. 2. Dorin, J. R., Emslie, E., van Heyningen, V. Related calcium-binding proteins map to the same subregion of chromosome 1q and to an extended region of synteny on mouse chromosome 3. Genomics 8: 420-426, 1990. 3. Engelkamp, D., Schafer, B. W., Erne, P., Heizmann, C. W. S100-alpha, CAPL, and CACY: molecular cloning and expression analysis of three calcium-binding proteins from human heart. Biochemistry 31: 10258-10264, 1992.

Uniprot ID: P26447

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 10-12 kDa

Calculated Molecular Weight: 123799 MW

Gene ID: 6275

Protein Name: Protein S100-A4

Gene Full Name: S100 calcium binding protein A4

Synonyms: 18A2; 42A; Calvasculin; CAPL; Fibroblast Specific Protein; Fibroblast-specific protein 1; FSP1; FSP-1; Metastasin; MTS1; P9KA; PEL98; Protein Mts1; Protein S100 A4; S100A4