Anti-RPL27 Antibody Picoband®

SKU: A09736-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RPL27

Specificity:

Background: 60S ribosomal protein L27 is a protein that in humans is encoded by the RPL27 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L27e family of ribosomal proteins and a component of the 60S subunit. A splice site mutation in this gene has been identified in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Immunogen: E.coli-derived human RPL27 recombinant protein (Position: M1-R108). Human RPL27 shares 100% amino acid (aa) sequence identity with both mouse and rat RPL27.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gallagher, R. A., McClean, P. M., Malik, A. N. Cloning and nucleotide sequence of a full length cDNA encoding ribosomal protein L27 from human fetal kidney. Biochim. Biophys. Acta 1217: 329-332, 1994. 2. Sim, E. U. H., Ang, C. H., Ng, C. C., Lee, C. W., Narayanan, K. Differential expression of a subset of ribosomal protein genes in cell lines derived from human nasopharyngeal epithelium. J. Hum. Genet. 55: 118-120, 2010. 3. Wang, R., Yoshida, K., Toki, T., Sawada, T., Uechi, T., Okuno, Y., Sato-Otsubo, A., Kudo, K., Kamimaki, I., Kanezaki, R., Shiraishi, Y., Chiba, K., and 21 others. Loss of function mutations in RPL27 and RPS27 identified by whole exome sequencing in Diamond-Blackfan anaemia. Brit. J. Haemat. 168: 854-864, 2015.

Uniprot ID: P61353

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 16 kDa

Calculated Molecular Weight: 16 kDa

Gene ID: 6155

Protein Name: Large ribosomal subunit protein eL27

Gene Full Name: ribosomal protein L27

Synonyms: RPL27; 60S ribosomal protein L27; Large ribosomal subunit protein eL27