Anti-RPL24 Antibody Picoband®

SKU: A07251-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC/IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: RPL24

Specificity:

Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L24E family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as ribosomal protein L30 because the encoded protein shares amino acid identity with the L30 ribosomal proteins from S. cerevisiae; however, its official name is ribosomal protein L24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Immunogen: E.coli-derived human RPL24 recombinant protein (Position: M1-R157). Human EIF3H shares 100% amino acid (aa) sequence identity with both mouse and rat RPL24.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Barkic, M., Crnomarkovic, S., Grabusic, K., Bogetic, I., Panic, L., Tamarut, S., Cokaric, M., Jeric, I., Vidak, S., Volarevic, S. The p53 tumor suppressor causes congenital malformations in Rpl24-deficient mice and promotes their survival. Molec. Cell. Biol. 29: 2489-2504, 2009. 2. Barna, M., Pusic, A., Zollo, O., Costa, M., Kondrashov, N., Rego, E., Rao, P. H., Ruggero, D. Suppression of Myc oncogenic activity by ribosomal protein haploinsufficiency. Nature 456: 971-975, 2008. 3. Johnson, K. R. Characterization of cDNA clones encoding the human homologue of Saccharomyces cerevisiae ribosomal protein L30. Gene 123: 283-285, 1993.

Uniprot ID: P83731

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 18 kDa

Calculated Molecular Weight: 18 kDa

Gene ID: 6152

Protein Name: Large ribosomal subunit protein eL24

Gene Full Name: ribosomal protein L24

Synonyms: ribosomal protein L24; Large ribosomal subunit protein eL24; 60S ribosomal protein L30; 60S ribosomal protein L24