Anti-RNA polymerase II/POLR2A Antibody Picoband®

SKU: A01029-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Monkey,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: POLR2A

Specificity: No cross reactivity with other proteins.

Background: DNA-ed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that in humans is encoded by the POLR2A gene. It is mapped to 17p13.1. This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Immunogen: E.coli-derived human RNA polymerase II/POLR2A recombinant protein (Position: D10-E321).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Egloff, S., O'Reilly, D., Chapman, R. D., Taylor, A., Tanzhaus, K., Pitts, L., Eick, D., Murphy, S. Serine-7 of the RNA polymerase II CTD is specifically required for snRNA gene expression. Science 318: 1777-1779, 2007. 2. Gnatt, A. L., Cramer, P., Fu, J., Bushnell, D. A., Kornberg, R. D. Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 angstrom resolution. Science 292: 1876-1882, 2001. 3. Haijes, H. A., Koster, M. J. E., Rehmann, H., Li, D., Hakonarson, H., Cappuccio, G., Hancarova, M., Lehalle, D., Reardon, W., Schaefer, G. B., Lehman, A., van de Laar, I. M. B. H., and 32 others. De novo heterozygous POLR2A variants cause a neurodevelopmental syndrome with profound infantile-onset hypotonia. Am. J. Hum. Genet. 105: 283-301, 2019.

Uniprot ID: P24928

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 220 kDa

Calculated Molecular Weight:

Gene ID: 5430

Protein Name: DNA-directed RNA polymerase II subunit RPB1

Gene Full Name: RNA polymerase II subunit A

Synonyms: hRPB220; hsRPB1; Pol II; POLR2; POLR2A; POLRA; RNA polymerase II subunit B1; RPB1; RPBh1; RpIILS; RPO2; RPOL2