Anti-Ribonuclease A/RNASE1 Antibody Picoband®

SKU: PA1507

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: RNASE1

Specificity: No cross reactivity with other proteins.

Background: RIBONUCLEASE A FAMILY, 1 (Ribonuclease pancreatic) is an enzyme that in humans is encoded by the RNASE1 gene. By genomic sequence analysis, RNASE1 gene is mapped the to chromosome 14q11.2, where it is linked to 7 other RNase A superfamily genes. Monomeric human pancreatic RNase, devoid of any biological activity other than its RNA degrading ability, was engineered into a dimeric protein with a cytotoxic action on mouse and human tumor cells, but lacking any appreciable toxicity on mouse and human normal cells. This dimeric variant of human pancreas RNase selectively sensitizes to apoptotic death cells derived from a human thyroid tumor. Because of its selectivity for tumor cells, and because of its human origin, this protein represents a potentially very attractive, novel tool for anticancer therapy.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Ribonuclease A.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Zhang, J., Dyer, K. D., Rosenberg, H. F. RNase 8, a novel RNase A superfamily ribonuclease expressed uniquely in placenta. Nucleic Acids Res. 30: 1169-1175, 2002. 2. Piccoli, R., Di Gaetano, S., De Lorenzo, C., Grauso, M., Monaco, C., Spalletti-Cernia, D., Laccetti, P., Cinatl, J., Matousek, J., D'Alessio, G. A dimeric mutant of human pancreatic ribonuclease with selective cytotoxicity toward malignant cells. Proc. Nat. Acad. Sci. 96: 7768-7773, 1999.

Uniprot ID: P07998

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 18 kDa

Calculated Molecular Weight: 17644 MW

Gene ID: 6035

Protein Name: Ribonuclease pancreatic

Gene Full Name: ribonuclease A family member 1, pancreatic

Synonyms: ribonuclease A family member 1; pancreatic; RAC1; RIB1; RNS1; RNASE1