Anti-Rex1/ZFP42 Antibody Picoband®

SKU: A08177-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: ZFP42

Specificity: No cross reactivity with other proteins.

Background: Rex1 is a protein that in humans is encoded by the ZFP42 gene. It is mapped to 4q35.2. Rex1 (Zfp-42) is a known marker of pluripotency, and is usually found in undifferentiated embryonic stem cells. In addition to being a marker for pluripotency, its regulation is also critical in maintaining a pluripotent state. As the cells begin to differentiate, Rex1 is severely and abruptly downregulated.

Immunogen: E.coli-derived human Rex1/ZFP42 recombinant protein (Position: A25-K310).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Gontan, C., Achame, E. M., Demmers, J., Barakat, T. S., Rentmeester, E., van IJcken, W., Grootegoed, J. A., Gribnau, J. RNF12 initiates X-chromosome inactivation by targeting REX1 for degradation. Nature 485: 386-390, 2012. 2. Henderson, J. K., Draper, J. S., Baillie, H. S., Fishel, S., Thomson, J. A., Moore, H., Andrews, P. W. Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337, 2002. 3. Hosler, B. A., LaRosa, G. J., Grippo, J. F., Gudas, L. J. Expression of REX-1, a gene containing zinc finger motifs, is rapidly reduced by retinoic acid in F9 teratocarcinoma cells. Molec. Cell. Biol. 9: 5623-5629, 1989.

Uniprot ID: Q96MM3

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 45 kDa

Calculated Molecular Weight:

Gene ID: 132625

Protein Name: Zinc finger protein 42 homolog

Gene Full Name: ZFP42 zinc finger protein

Synonyms: ZFP42 zinc finger protein; REX-1; REX1; ZNF754; zfp-42