Anti-REN/Renin Antibody

SKU: A03689-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IHC

Application Details: Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human

Gene Name: REN

Specificity:

Background: This gene encodes renin, an aspartic protease that is secreted by the kidneys. Renin is a part of the renin-angiotensin-aldosterone system involved in regulation of blood pressure, and electrolyte balance. This enzyme catalyzes the first step in the activation pathway of angiotensinogen by cleaving angiotensinogen to form angiotensin I, which is then converted to angiotensin II by angiotensin I converting enzyme. This cascade can result in aldosterone release, narrowing of blood vessels, and increase in blood pressure as angiotension II is a vasoconstrictive peptide. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause hyperuricemic nephropathy familial juvenile 2, familial hyperproreninemia, and renal tubular dysgenesis.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human REN/Renin, which shares 95% and 95% amino acid (aa) sequence identity with mouse and rat REN/Renin, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Abel, K. J., Gross, K. W. Close physical linkage of the murine Ren-1 and Ren-2 loci. Nucleic Acids Res. 16: 2111-2126, 1988. 2. Caron, K. M. I., James, L. R., Kim, H.-S., Knowles, J., Uhlir, R., Mao, L., Hagaman, J. R., Cascio, W., Rockman, H., Smithies, O. Cardiac hypertrophy and sudden death in mice with a genetically clamped renin transgene. Proc. Nat. Acad. Sci. 101: 3106-3111, 2004. 3. Chirgwin, J. M., Schaefer, I. M., Diaz, J. A., Lalley, P. A. Mouse kidney renin gene is on chromosome one. Somat. Cell Molec. Genet. 10: 633-637, 1984.

Uniprot ID: P00797

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 53-60 kDa

Calculated Molecular Weight: 28461 MW

Gene ID: 5972

Protein Name: Renin

Gene Full Name: renin

Synonyms: Angiotensinogenase; REN; Renin