Anti-RASAL3 Antibody Picoband®

SKU: A11713-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: RASAL3

Specificity:

Background: This gene belongs to the Ras GTPase-activating proteins (RasGAP) family and encodes a protein with pleckstrin homology (PH), C2, and Ras GTPase-activation protein (RasGAP) domains. This protein is localized near or at the plasma membrane when expressed exogenously. Reduced expression of this gene in some cell lines resulted in increased levels of the active form of Ras (Ras-GTP), suggesting that this gene may play a role in negatively regulating the Ras signaling pathway. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen: E.coli-derived human RASAL3 recombinant protein (Position: R48-Q925). Human RASAL3 shares 82.4% amino acid (aa) sequence identity with mouse RASAL3.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gross, M. B. Personal Communication. Baltimore, Md. 9/18/2015. 2. Saito, S., Kawamura, T., Higuchi, M., Kobayashi, T., Yoshita-Takahashi, M., Yamazaki, M., Abe, M., Sakimura, K., Kanda, Y., Kawamura, H., Jiang, S., Naito, M., Yoshizaki, T., Takahashi, M., Fujii, M. RASAL3, a novel hematopoietic RasGAP protein, regulates the number and functions of NKT cells. Europ. J. Immun. 45: 1512-1523, 2015.

Uniprot ID: Q86YV0

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 112 kDa

Calculated Molecular Weight: 112 kDa

Gene ID: 64926

Protein Name: RAS protein activator like-3

Gene Full Name: RAS protein activator like 3

Synonyms: RAS protein activator like-3; RAS protein activator like 3