Anti-Rad9b Antibody Picoband®

SKU: A13507-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: ELISA,Flow Cytometry,IHC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5μg/ml, -

Gene Name: Rad9b

Specificity:

Background: By coimmunoprecipitation of erythroleukemia cells transfected with RAD9B, it was found that RAD9B associated with 3 endogenous checkpoint proteins, HUS1, RAD1, and RAD17, possibly in a 9-1-1-like complex. RAD9B also interacted with transfected HUS1B. It was also found decreased abundance of RAD9B mRNA in testicular seminomas relative to normal tissue controls and other types of testicular tumors.

Immunogen: E.coli-derived mouse Rad9b recombinant protein (Position: M1-G403).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Dufault, V. M., Oestreich, A. J., Vroman, B. T., Karnitz, L. M. Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene. Genomics 82: 644-651, 2003. 2. Hopkins, K. M., Wang, X., Berlin, A., Hang, H., Thaker, H. M., Lieberman, H. B. Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue. Cancer Res. 63: 591-598, 2003.

Uniprot ID: Q6WBX7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 60 kDa

Calculated Molecular Weight: 64099 MW

Gene ID: 231724

Protein Name: Cell cycle checkpoint control protein RAD9B

Gene Full Name: RAD9 checkpoint clamp component B

Synonyms: RAD9 checkpoint clamp component B; Rad9b