Anti-RAB27A Antibody Picoband® (monoclonal, 2F5)

SKU: M01608

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells

Gene Name: RAB27A

Specificity: No cross reactivity with other proteins.

Background: Ras-related protein Rab-27A is a protein that in humans is encoded by the RAB27A gene. The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

Immunogen: E. coli-derived human RAB27A recombinant protein (Position: L98-K216).

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Reference: 1. Aksu, G., Kutukculer, N., Genel, F., Vergin, C., Omowaire, B. Griscelli syndrome without hemophagocytosis in an eleven-year-old girl: expanding the phenotypic spectrum ofRab27A mutations in humans. Am. J. Med. Genet. 116A: 329-333, 2003. 2. Kuroda, T. S., Fukuda, M., Ariga, H., Mikoshiba, K. The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem. 277: 9212-9218, 2002.

Uniprot ID: P51159

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 2F5

Observed Molecular Weight: 27 kDa

Calculated Molecular Weight:

Gene ID: 5873

Protein Name: Ras-related protein Rab-27A

Gene Full Name: RAB27A, member RAS oncogene family

Synonyms: GS2; GTP binding protein Ram; HsT18676; Rab 27; RAB27; RAB27A; RAM; Ras related protein Rab 27A