Anti-PYGM Antibody Picoband®

SKU: A02971-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: PYGM

Specificity:

Background: This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human PYGM recombinant protein (Position: V390-R804). Human PYGMshares 95.9% and 94.2% amino acid (aa) sequence identity with mouse and rat PYGM, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Andreu, A. L., Nogales-Gadea, G., Cassandrini, D., Arenas, J., Bruno, C. McArdle disease: molecular genetic update. Acta Myol. 26: 53-57, 2007. 2. Bartram, C., Edwards, R. H. T., Clague, J., Beynon, R. J. McArdle's disease: a nonsense mutation in exon 1 of the muscle glycogen phosphorylase gene explains some but not all cases. Hum. Molec. Genet. 2: 1291-1293, 1993. 3. Bruno, C., Lofberg, M., Tamburino, L., Jankala, H., Hadjigeorgiou, G. M., Andreu, A. L., Shanske, S., Somer, H., DiMauro, S. Molecular characterization of McArdle's disease in two large Finnish families. J. Neurol. Sci. 165: 121-125, 1999.

Uniprot ID: P11217

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 97 kDa

Calculated Molecular Weight: 97 kDa

Gene ID: 5837

Protein Name: Glycogen phosphorylase, muscle form

Gene Full Name: glycogen phosphorylase, muscle associated

Synonyms: PYGM; Myophosphorylase; EC:2.4.1.1