Anti-PYCR2 Antibody Picoband®

SKU: A10327-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PYCR2

Specificity:

Background: Pyrroline-5-carboxylate reductase family, member 2 is a protein that in humans is encoded by the PYCR2 gene. This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene.

Immunogen: E.coli-derived human PYCR2 recombinant protein (Position: Q10-D320).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. De Ingeniis, J., Ratnikov, B., Richardson, A. D., Scott, D. A., Aza-Blanc, P., De, S. K., Kazanov, M., Pellecchia, M., Ronai, Z., Osterman, A. L., Smith, J. W. Functional specialization in proline biosynthesis of melanoma. PLoS One 7: e45190, 2012. Note: Electronic Article. 2. Escande-Beillard, N., Loh, A., Saleem, S. N., Kanata, K., Hashimoto, Y., Altunoglu, U., Metoska, A., Grandjean, J., Ng, F. M., Pomp, O., Baburajendran, N., Wong, J., Hill, J., Beillard, E., Cozzone, P., Zaki, M., Kayserili, H., Hamada, H., Shiratori, H., Reversade, B. Loss of PYCR2 causes neurodegeneration by increasing cerebral glycine levels via SHMT2. Neuron 107: 82-94, 2020. 3. Nakayama, T., Al-Maawali, A., El-Quessny, M., Rajab, A., Khalil, S., Stoler, J. M., Tan, W.-H., Nasir, R., Schmitz-Abe, K., Hill, R. S., Partlow, J. N., Al-Saffar, M., and 10 others. Mutations in PYCR2, encoding pyrroline-5-carboxylate reductase 2, cause microcephaly and hypomyelination. Am. J. Hum. Genet. 96: 709-719, 2015.

Uniprot ID: Q96C36

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight: 41409 MW

Gene ID: 29920

Protein Name: Pyrroline-5-carboxylate reductase 2

Gene Full Name: pyrroline-5-carboxylate reductase 2

Synonyms: P5C reductase 2; P5CR 2; P5CR2; PYCR2