Anti-PTEN Antibody Picoband®

SKU: A00006-5

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat

Gene Name: PTEN

Specificity:

Background: Phosphatase and tensin homolog?(PTEN) is a phosphatase in humans and is encoded by the?PTEN?gene. This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PTEN identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Agrawal, S., Pilarski, R., Eng, C. Different splicing defects lead to differential effects downstream of the lipid and protein phosphatase activities of PTEN. Hum. Molec. Genet. 14: 2459-2468, 2005. 2. Alimonti, A., Carracedo, A., Clohessy, J. G., Trotman, L. C., Nardella, C., Egia, A., Salmena, L., Sampieri, K., Haveman, W. J., Brogi, E., Richardson, A. L., Zhang, J., Pandolfi, P. P. Subtle variations in Pten dose determine cancer susceptibility. Nature Genet. 42: 454-458, 2010. 3. Alvarez-Breckenridge, C. A., Waite, K. A., Eng, C. PTEN regulates phospholipase D and phospholipase C. Hum. Molec. Genet. 16: 1157-1163, 2007.

Uniprot ID: P60484

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 56 kDa

Calculated Molecular Weight: 47,65,20 kDa

Gene ID: 5728

Protein Name: Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN

Gene Full Name: phosphatase and tensin homolog

Synonyms: 10q23del; Bannayan Zonana syndrome; BC005821; BZS; ITGA 2; MGC11227; MHAM; MMAC1; Multiple hamartoma (Cowden syndrome); phosphatase and tensin homolog; PTEN; PTEN1; TEP1