Anti-PRKAG2 Antibody Picoband®

SKU: A02758-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PRKAG2

Specificity:

Background: 5'-AMP-activated protein kinase subunit gamma-2 is an enzyme that in humans is encoded by the PRKAG2 gene. AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Immunogen: E.coli-derived human PRKAG2 recombinant protein (Position: K24-H530).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Akman, H. O., Sampayo, J. N., Ross, F. A., Scott, J. W., Wilson, G., Benson, L., Bruno, C., Shanske, S., Hardie, D. G., DiMauro, S. Fatal infantile cardiac glycogenosis with phosphorylase kinase deficiency and a mutation in the gamma-2-subunit of AMP-activated protein kinase. Pediat. Res. 62: 499-504, 2007. 2. Arad, M., Benson, D. W., Perez-Atayde, A. R., McKenna, W. J., Sparks, E. A., Kanter, R. J., McGarry, K., Seidman, J. G., Seidman, C. E. Constitutively active AMP kinase mutations cause glycogen storage disease mimicking hypertrophic cardiomyopathy. J. Clin. Invest. 109: 357-362, 2002. 3. Arad, M., Maron, B. J., Gorham, J. M., Johnson, W. H., Jr., Saul, J. P., Perez-Atayde, A. R., Spirito, P., Wright, G. B., Kanter, R. J., Seidman, C. E., Seidman, J. G. Glycogen storage diseases presenting as hypertrophic cardiomyopathy. New Eng. J. Med. 352: 362-372, 2005.

Uniprot ID: Q9UGJ0

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 63 kDa

Calculated Molecular Weight: 12252 MW

Gene ID: 51422

Protein Name: 5'-AMP-activated protein kinase subunit gamma-2

Gene Full Name: protein kinase AMP-activated non-catalytic subunit gamma 2

Synonyms: AAKG; AAKG2; AMPK gamma2; AMPK subunit gamma 2; CMH6; H91620p; PRKAG2; WPWS