Anti-PREX2 Antibody Picoband®

SKU: A06715-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PREX2

Specificity:

Background: Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 is a protein that in humans is encoded by the PREX2 gene. The protein encoded by this gene belongs to the phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger (PREX) family, which are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. Structural domains of this protein include the catalytic diffuse B-cell lymphoma homology and pleckstrin homology (DHPH) domain, two disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ domains, and a C-terminal inositol polyphosphate-4 phosphatase (IP4P) domain that is found in one of the isoforms. This protein facilitates the exchange of GDP for GTP on Rac1, allowing the GTP-bound Rac1 to activate downstream effectors. Studies also show that the pleckstrin homology domain of this protein interacts with the phosphatase and tensin homolog (PTEN) gene product to inhibit PTEN phosphatase activity, thus activating the phosphoinositide-3 kinase (PI3K) signaling pathway. Conversely, the PTEN gene product has also been shown to inhibit the GEF activity of this protein. This gene plays a role in insulin-signaling pathways, and either mutations or overexpression of this gene have been observed in some cancers.

Immunogen: E.coli-derived human PREX2 recombinant protein (Position: D582-Q1094).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Berger, M. F., Hodis, E., Heffernan, T. P., Deribe, Y. L., Lawrence, M. S., Protopopov, A., Ivanova, E., Watson, I. R., Nickerson, E., Ghosh, P., Zhang, H., Zeid, R., and 36 others. Melanoma genome sequencing reveals frequent PREX2 mutations. Nature 485: 502-506, 2012. 2. Donald, S., Hill, K., Lecureuil, C., Barnouin, R., Krugmann, S., Coadwell, W. J., Andrews, S. R., Walker, S. A., Hawkins, P. T., Stephens, L. R., Welch, H. C. E. P-Rex2, a new guanine-nucleotide exchange factor for Rac. FEBS Lett. 572: 172-176, 2004. 3. Donald, S., Humby, T., Fyfe, I., Segonds-Pichon, A., Walker, S. A., Andrews, S. R., Coadwell, W. J., Emson, P., Wilkinson, L. S., Welch, H. C. E. P-Rex2 regulates Purkinje cell dendrite morphology and motor coordination. Proc. Nat. Acad. Sci. 105: 4483-4488, 2008.

Uniprot ID: Q70Z35

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 183 kDa

Calculated Molecular Weight: 104553 MW

Gene ID: 80243

Protein Name: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein

Gene Full Name: phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2

Synonyms: DEP.2; DEPDC2; P REX2; PREX2