Anti-POLR1A Antibody Picoband®

SKU: A10549-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: POLR1A

Specificity:

Background: DNA-directed RNA polymerase I subunit RPA1 is an enzyme that in humans is encoded by the POLR1A gene. The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis.

Immunogen: E.coli-derived human POLR1A recombinant protein (Position: E607-R1720). Human POLR1A shares 86.3% and 87.3% amino acid (aa) sequence identity with mouse and rat POLR1A, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. da Rocha, L. A., Pires, L. V. L., Yamamoto, G. L., Magliocco Ceroni, J. R., Honjo, R. S., de Novaes Franca Bisneto, E., Oliveira, L. A. N., Rosenberg, C., Krepischi, A. C. V., Passos-Bueno, M. R., Kim, C. A., Bertola, D. R. Congenital limb deficiency: genetic investigation of 44 individuals presenting mainly longitudinal defects in isolated or syndromic forms. Clin. Genet. 100: 615-623, 2021. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 6/2/2015. 3. Kara, B., Koroglu, C., Peltonen, K., Steinberg, R., Maras Genc, H., Holtta-Vuori, M., Guven, A., Kanerva, K., Kotil, T., Solakoglu, S., Zhou, Y., Olkkonen, V. M., Ikonen, E., Laiho, M., Tolun, A. Severe neurodegenerative disease in brothers with homozygous mutation in POLR1A. Europ. J. Hum. Genet. 25: 315-323, 2017.

Uniprot ID: O95602

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 195 kDa

Calculated Molecular Weight: 195 kDa

Gene ID: 25885

Protein Name: DNA-directed RNA polymerase I subunit RPA1

Gene Full Name: RNA polymerase I subunit A

Synonyms: A190; POLR1A; RNA polymerase I subunit A1; RPA1; RPA194; RPO1 4