Anti-POLD3 Antibody Picoband®

SKU: A05111-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: POLD3

Specificity:

Background: DNA polymerase delta subunit 3 is an enzyme that in humans is encoded by the POLD3 gene. This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene.

Immunogen: E.coli-derived human POLD3 recombinant protein (Position: D11-K296). Human POLD3 shares 87.8% amino acid (aa) sequence identity with mouse POLD3.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Dilley, R. L., Verma, P., Cho, N. W., Winters, H. D., Wondisford, A. R., Greenberg, R. A. Break-induced telomere synthesis underlies alternative telomere maintenance. Nature 539: 54-58, 2016. 2. Hughes, P., Tratner, I., Ducoux, M., Piard, K., Baldacci, G. Isolation and identification of the third subunit of mammalian DNA polymerase delta by PCNA-affinity chromatography of mouse FM3A cell extracts. Nucleic Acids Res. 27: 2108-2114, 1999. 3. Liu, G., Warbrick, E. The p66 and p12 subunits of DNA polymerase delta are modified by ubiquitin and ubiquitin-like proteins. Biochem. Biophys. Res. Commun. 349: 360-366, 2006.

Uniprot ID: Q15054

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 70 kDa

Calculated Molecular Weight:

Gene ID: 10714

Protein Name: DNA polymerase delta subunit 3

Gene Full Name: DNA polymerase delta 3, accessory subunit

Synonyms: POLD3; KIAA0039; DNA polymerase delta subunit 3; DNA polymerase delta subunit C; DNA polymerase delta subunit p66; DNA polymerase delta subunit p68