Anti-PLS1 Antibody Picoband®

SKU: A07675-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PLS1

Specificity:

Background: Fimbrin also known as is plastin 1 is a protein that in humans is encoded by the PLS1 gene. Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11.

Immunogen: E.coli-derived human PLS1 recombinant protein (Position: E14-L581). Human PLS1 shares 93.8% amino acid (aa) sequence identity with mouse PLS1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Diaz-Horta, O., Bademci, G., Tokgoz-Yilmaz, S., Guo, S., Zafeer, F., Sineni, C. J., Duman, D., Farooq, A., Tekin, M. Novel variant pE269K confirms causative role of PLA1 mutations in autosomal dominant hearing loss. Clin. Genet. 96: 575-578, 2019. 2. Grimm-Gunter, E-M. S., Revenu, C., Ramos, S., Hurbain, I., Smyth, N., Ferrary, E., Louvard, D., Robine, S., Rivero, F. Plastin 1 binds to keratin and is required for terminal web assembly in the intestinal epithelium. Molec. Biol. Cell 20: 2549-2562, 2009. 3. Lin, C.-S., Shen, W., Chen, Z. P., Tu, Y.-H., Matsudaira, P. Identification of I-plastin, a human fimbrin isoform expressed in intestine and kidney. Molec. Cell. Biol. 14: 2457-2467, 1994.

Uniprot ID: Q14651

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 70 kDa

Calculated Molecular Weight: 70 kDa

Gene ID: 5357

Protein Name: Plastin-1

Gene Full Name: plastin 1

Synonyms: PLS1; Plastin-1; Intestine-specific plastin; I-plastin