Anti-PKR/EIF2AK2 Antibody Picoband®

SKU: PB9803

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: EIF2AK2

Specificity: No cross reactivity with other proteins.

Background: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2), also called PKR, is an enzyme that in humans is encoded by the EIF2AK2 gene. Activation of EIF2AK2 allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2, leading to the inhibition of protein synthesis. By FISH analysis, Squire et al. (1993) assigned the EIF2AK2 gene to the boundary between chromosome 2p22-p21. Ben-Asouli et al. (2002) showed that human gamma-interferon mRNA uses local activation of PKR in the cell to control its own translation yield. IFNG mRNA was found to activate PKR through a pseudoknot in its 5-prime untranslated region. Taylor et al. (1999) studied the mechanism underlying the resistance of hepatitis C virus (HCV) to interferon. They demonstrated that the HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PKR and the translation initiation factor EIF2-alpha, a target of PKR. E2 inhibited the kinase activity of PKR and blocked its inhibitory effect on protein synthesis and cell growth.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PKR, different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by thirteen amino acids.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Ben-Asouli, Y., Banai, Y., Pel-Or, Y., Shir, A., Kaempfer, R. Human interferon-gamma mRNA autoregulates its translation through a pseudoknot that activates the interferon-inducible protein kinase PKR. Cell 108: 221-232, 2002. 2. Kuhen, K. L., Shen, X., Carlisle, E. R., Richardson, A.L., Weier, H.-U. G., Tanaka, H., Samuel, C. E. Structural organization of the human gene (PKR) encoding an interferon-inducible RNA-dependent protein kinase (PKR) and differences from its mouse homolog. Genomics 36: 197-201, 1996. 3. Squire, J., Meurs, E. F., Chong, K. L., McMillan, N. A. J., Hovanessian, A. G., Williams, B. R. G. Localization of the human interferon-induced, ds-RNA activated p68 kinase gene (PRKR) to chromosome 2p21-p22. Genomics 16: 768-770, 1993. 4. Taylor, D. R., Shi, S. T., Romano, P. R., Barber, G. N., Lal, M. M. C. Inhibition of the interferon-inducible protein kinase PKR by HCV E2 protein. Science 285: 107-110, 1999.

Uniprot ID: P19525

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 69 kDa

Calculated Molecular Weight: 62094 MW

Gene ID: 5610

Protein Name: Interferon-induced, double-stranded RNA-activated protein kinase

Gene Full Name: eukaryotic translation initiation factor 2 alpha kinase 2

Synonyms: eIF 2A protein kinase 2; EIF2AK1; EIF2AK2; P1/eIF 2A protein kinase; p68 kinase; PKR; PRKR; Protein kinase RNA activated