Anti-PKC theta/PRKCQ Antibody Picoband®

SKU: A01293-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: PRKCQ

Specificity:

Background: Protein kinase C theta (PKC-θ) is an enzyme that in humans is encoded by the PRKCQ gene. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.

Immunogen: E.coli-derived human PKC theta/PRKCQ recombinant protein (Position: E23-S706).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Baier, G., Telford, D., Giampa, L., Coggeshall, K. M., Baier-Bitterlich, G., Isakov, N., Altman, A. Molecular cloning and characterization of PKC-theta, a novel member of the protein (PKC) gene family expressed predominantly in hematopoietic cells. J. Biol. Chem. 268: 4997-5004, 1993. 2. Belguise, K., Sonenshein, G. E. PKC-theta promotes c-Rel-driven mammary tumorigenesis in mice and humans by repressing estrogen receptor-alpha synthesis. J. Clin. Invest. 117: 4009-4021, 2007. 3. Blanco, G., Brown, S. D. M. Genetic mapping of protein kinase C theta (Pkcq) to mouse chromosome 2. Mammalian Genome 8: 70-71, 1997.

Uniprot ID: Q04759

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 78 kDa

Calculated Molecular Weight:

Gene ID: 5588

Protein Name: Protein kinase C theta type

Gene Full Name: protein kinase C theta

Synonyms: protein kinase C theta; PRKCT; nPKC-theta; PRKCQ