Anti-Pirh2/RCHY1 Antibody Picoband®

SKU: A04533-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RCHY1

Specificity:

Background: RING finger and CHY zinc finger domain-containing protein 1 is a protein that in humans is encoded by the RCHY1 gene. The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Immunogen: E.coli-derived human Pirh2/RCHY1 recombinant protein (Position: Q17-Q250).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Fedorova, O. , Gudovich, A. , Daks, A. , Baidyuk, E. , & Barlev, N. A. . (2021). Regulation of autophagy flux by e3 ubiquitin ligase pirh2 in lung cancer. Biochemical and Biophysical Research Communications, 563, 119-125. 2. Yifeng, Bao, Xue, Wu, Debin, & Yuan, et al. (2017). High expression of pirh2 is associated with poor prognosis in glioma. Cellular and molecular neurobiology. 3. Yang, ShuyunChen, YingyingSun, FulanNi, QichaoWang, HuaHuang, YeqingZhang, ChunhuiLiu, KunWang, ShuoQiu, JianXu, ZhenHua, LuHe, Zhixian. (2016). Downregulated pirh2 can decrease the proliferation of breast cancer cells. Archives of medical research, 47(3).

Uniprot ID: Q96PM5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 21 kDa

Calculated Molecular Weight:

Gene ID: 25898

Protein Name: RING finger and CHY zinc finger domain-containing protein 1

Gene Full Name: ring finger and CHY zinc finger domain containing 1

Synonyms: ARNIP; CHIMP; DKFZp586C1620; hARNIP; hPirh2; p53 induced RING H2 protein; PIRH2; PRO1996; RCHY1; RING finger protein 199; RNF199; Zinc finger protein 363; ZNF363