Anti-PINX1 Antibody Picoband®

SKU: PB9498

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: PINX1

Specificity: No cross reactivity with other proteins.

Background: PINX1, also known as PIN2 interacting protein 1, is a telomerase inhibitor and a possible tumor suppressor. It is mapped to 8p23. Over-expression of PINX1 results in decreased telomerase activity, telomere shortening, and induction of crisis. Reduction of PINX1 leads to an increase in telomerase activity and elongation of telomeres. PINX1 differs from other proteins that regulate telomere length in that it acts on telomerase while other proteins adjust telomere length without affecting telomerase activity. The PINX1 budding yeast orthologue Gnop1 inhibits telomerase by isolating the uncomplexed TERT protein so that it cannot associate with the telomerase template RNA, which prevents telomerase from being assembled. However, in humans, PINX1 impedes already assembled telomerase.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PINX1, different from the related mouse and rat sequences by three amino acids.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Banik, S.S.R.; Counter, C.M. (September 2004). Characterization of Interactions between PinX1 and Human Telomerase Subunits hTERT and hTR. Journal of Biological Chemistry. 2. Zhou, X Z; Lu K P (Nov 2001). The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor. Cell (United States) 107 (3): 347–59.

Uniprot ID: Q96BK5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 37 kDa

Calculated Molecular Weight: 37035 MW

Gene ID: 54984

Protein Name: PIN2/TERF1-interacting telomerase inhibitor 1

Gene Full Name: PIN2 (TERF1) interacting telomerase inhibitor 1

Synonyms: LPTL; LPTS; PIN2 interacting protein 1; Pin2 interacting protein X1; PINX1; Protein 67 11 3; TRF1 interacting protein 1