Anti-PHEX Antibody Picoband®

SKU: A02078

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: PHEX

Specificity: No cross reactivity with other proteins.

Background: Phosphate-regulating neutral endopeptidase, X-linked also known as phosphate-regulating gene with homologies to endopeptidases on the X chromosome or metalloendopeptidase homolog PEX is an enzyme that in humans is encoded by the PHEX gene. It is mapped to Xp22.11. The protein encoded by this gene is a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein is thought to be involved in bone and dentin mineralization and renal phosphate reabsorption. Mutations in this gene cause X-linked hypophosphatemic rickets. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human PHEX recombinant protein (Position: E145-N718).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Christie, P. T., Harding, B., Nesbit, M. A., Whyte, M. P., Thakker, R. V. X-linked hypophosphatemia attributable to pseudoexons of the PHEX gene. J. Clin. Endocr. Metab. 86: 3840-3844, 2001. 2. Dixon, P. H., Christie, P. T., Wooding, C., Trump, D., Grieff, M., Holm, I., Gertner, J. M., Schmidtke, J., Shah, B., Shaw, N., Smith, C., Tau, C., Schlessinger, D., Whyte, M. P., Thakker, R. V. Mutational analysis of PHEX gene in X-linked hypophosphatemia. J. Clin. Endocr. Metab. 83: 3615-3623, 1998. 3. Econs, M. J., Friedman, N. E., Rowe, P. S. N., Speer, M. C., Francis, F., Strom, T. M., Oudet, C., Smith, J. A., Ninomiya, J. T., Lee, B. E., Bergen, H. A PHEX gene mutation is responsible for adult-onset vitamin D-resistant hypophosphatemic osteomalacia: evidence that the disorder is not a distinct entity from X-linked hypophosphatemic rickets. J. Clin. Endocr. Metab. 83: 3459-3462, 1998.

Uniprot ID: P78562

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 100-110 kDa

Calculated Molecular Weight: 86 kDa

Gene ID: 5251

Protein Name: Phosphate-regulating neutral endopeptidase PHEX

Gene Full Name: phosphate regulating endopeptidase X-linked

Synonyms: HPDR; HPDR1; HYP; HYP1; LXHR; PEX; XLH; PHEX