Anti-PGRMC1 Antibody Picoband®

SKU: PB9775

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IF,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunofluorescence, 5 μg/ml, Human

Gene Name: PGRMC1

Specificity: No cross reactivity with other proteins.

Background: Progesterone receptor membrane component 1 (PGRMC1) is a protein which co-purifies with progesterone binding proteins in the liver and ovary. In humans, the PGRMC1 protein is encoded by the PGRMC1 gene. The Sigma-2 receptor was recently identified as potentially being the same as PGRMC1. The sole biochemical function of PGRMC1 is heme-binding. PGRMC1 shares key structural motifs with cytochrome b5. It binds and activates P450 proteins, which are important in drug, hormone and lipid metabolism. Also, PGRMC1 binds to PAIR-BP1 (plasminogen activator inhibitor RNA-binding protein-1). However, its expression outside of the reproductive tract and in males suggests multiple functions for the protein. These may include binding to Insig (insulin-induced gene), which regulates cholesterol synthesis.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PGRMC1, identical to the related mouse sequence, and different from the related rat sequence by two amino acids.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Gerdes D, Wehling M, Leube B, Falkenstein E (Jul 1998). Cloning and tissue expression of two putative steroid membrane receptors.Biological Chemistry 379 (7): 907–11. 2. Meyer C, Schmid R, Scriba PC, Wehling M (Aug 1996). Purification and partial sequencing of high-affinity progesterone-binding site(s) from porcine liver membranes. European Journal of Biochemistry / FEBS 239(3): 726–31. 3. Xu J, Zeng C, Chu W, Pan F, Rothfuss JM, Zhang F, Tu Z, Zhou D, Zeng D, Vangveravong S, Johnston F, Spitzer D, Chang KC, Hotchkiss RS, Hawkins WG, Wheeler KT, Mach RH (2011). Identification of the PGRMC1 protein complex as the putative sigma-2 receptor binding site. Nature Communications 2 (2): 380.

Uniprot ID: O00264

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 26 kDa

Calculated Molecular Weight: 21671 MW

Gene ID: 10857

Protein Name: Membrane-associated progesterone receptor component 1

Gene Full Name: progesterone receptor membrane component 1

Synonyms: HPR6.6; MPR; PGRMC; PGRMC1