Anti-Pea3/ETV4 Antibody Picoband®

SKU: PB9699

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Rat

Gene Name: ETV4

Specificity: No cross reactivity with other proteins.

Background: ETS translocation variant 4  (ETV4), also known as polyoma enhancer activator 3  (PEA3), or E1AF, is a member of the PEA3 subfamily of Ets transcription factors. It is mapped to 17q21.31. E1AF can activate the promoters of various matrix metalloproteinases, genes whose expression is associated with tumor cell invasion and metastasis, by 10 to 20 fold. Pea3 is detected in cells of epithelial and fibroblastic origin. It is also a transcriptional activator that binds to the enhancer of the adenovirus E1A gene.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Pea3, different from the related mouse sequence by seven amino acids.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Davidson B, Goldberg I, Tell L, et al. (2004). The clinical role of the PEA3 transcription factor in ovarian and breast carcinoma in effusions. Clin. Exp. Metastasis 21 (3): 191–9. 2. Higashino, F., Yoshida, K., Noumi, T., Seiki, M., Fujinaga, K. Ets-related protein E1A-F can activate three different matrix metalloproteinase gene promoters. Oncogene 10: 1461-1463, 1995. 3. Isobe M, Yamagishi F, Yoshida K, Higashino F, Fujinaga K (Feb 1996). Assignment of the ets-related transcription factor E1A-F gene (ETV4) to human chromosome region 17q21. Genomics 28 (2): 357–9.

Uniprot ID: P43268

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 68 kDa

Calculated Molecular Weight: 53938 MW

Gene ID: 2118

Protein Name: ETS translocation variant 4

Gene Full Name: ETS variant transcription factor 4

Synonyms: E1A F; E1AF; ETS translocation variant 4; ets variant 4; ETV4; PEA3; PEAS3; Protein PEA3