Anti-PDLIM7 Antibody Picoband®

SKU: A04377-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PDLIM7

Specificity:

Background: PDZ and LIM domain protein 7 is a protein that in humans is encoded by the PDLIM7 gene. The protein encoded by this gene is representative of a family of proteins composed of conserved PDZ and LIM domains. LIM domains are proposed to function in protein-protein recognition in a variety of contexts including gene transcription and development and in cytoskeletal interaction. The LIM domains of this protein bind to protein kinases, whereas the PDZ domain binds to actin filaments. The gene product is involved in the assembly of an actin filament-associated complex essential for transmission of ret/ptc2 mitogenic signaling. The biological function is likely to be that of an adapter, with the PDZ domain localizing the LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Alternative splicing of this gene results in multiple transcript variants.

Immunogen: E.coli-derived human PDLIM7 recombinant protein (Position: N275-V457).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bach, I. The LIM domain: regulation by association. Mech. Dev. 91: 5-17, 2000. 2. Durick, K., Gill, G. N., Taylor, S. S. Shc and Enigma are both required for mitogenic signaling by Ret/ptc2. Molec. Cell. Biol. 18: 2298-2308, 1998. 3. Guy, P. M., Kenny, D. A., Gill, G. N. The PDZ domain of the LIM protein enigma binds to beta-tropomyosin. Molec. Biol. Cell 10: 1973-1984, 1999.

Uniprot ID: Q9NR12

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50 kDa

Calculated Molecular Weight:

Gene ID: 9260

Protein Name: PDZ and LIM domain protein 7

Gene Full Name: PDZ and LIM domain 7

Synonyms: ENIGMA; LIM mineralization protein; LMP; PDLIM7; PDZ and LIM domain 7 (enigma); PDZ and LIM domain protein 7; Protein enigma