Anti-PCYT2 Antibody Picoband®

SKU: A07910-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC/IF,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: PCYT2

Specificity:

Background: This gene encodes an enzyme that catalyzes the formation of CDP-ethanolamine from CTP and phosphoethanolamine in the Kennedy pathway of phospholipid synthesis. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human PCYT2 recombinant protein (Position: D32-F389). Human PCYT2 shares 89.5% and 94.4% amino acid (aa) sequence identity with mouse and rat PCYT2, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Christen, M., Oevermann, A., Rupp, S., Vaz, F. M., Wever, E. J. M., Braus, B. K., Jagannathan, V., Kehl, A., Hytonen, M. K., Lohi, H., Leeb, T. PCYT2 deficiency in Saarlooswolfdogs with progressive retinal, central, and peripheral neurodegeneration. Molec. Genet. Metab. 141: 108149, 2024. 2. Fullerton, M. D., Hakimuddin, F., Bakovic, M. Developmental and metabolic effects of disruption of the mouse CTP:phosphoethanolamine cytidylyltransferase gene (Pcyt2). Molec. Cell Biol. 27: 3327-3336, 2007. 3. Kaiyrzhanov, R., Wortmann, S., Reid, T., Dehghani, M., Vahidi Mehrjardi, M. Y., Alhaddad, B., Wagner, M., Deschauer, M., Cordts, I., Fernandez-Murray, J. P., Treffer, V., Metanat, Z., Pittman, A., Houlden, H., Meitinger, T., Carroll, C., McMaster, C. R., Maroofian, R. Defective phosphatidylethanolamine biosynthesis leads to a broad ataxia-spasticity spectrum. Brain 144: e30, 2021. Note: Erratum: Brain 144: e52, 2021.

Uniprot ID: Q99447

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 44 kDa

Calculated Molecular Weight: 44 kDa

Gene ID: 5833

Protein Name: Ethanolamine-phosphate cytidylyltransferase

Gene Full Name: phosphate cytidylyltransferase 2, ethanolamine

Synonyms: Ethanolamine-phosphate cytidylyltransferase; Ethanolamine phosphate cytidylyltransferase; EC:2.7.7.14