Anti-PCNA Antibody (Monoclonal, PC 10)

SKU: MA1083

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IHC,ICC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, mouse, rat
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry (Frozen Section), 0.4-1μg/ml, Human, mouse, rat, -
Western blot, 2μg/ml, Human, mouse, rat

Gene Name: PCNA

Specificity: No cross reactivity with other proteins.

Background: Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.

Immunogen: Protein A fusion protein.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. Bravo, R. : Synthesis of the nuclear protein cyclin (PCNA) and its relationship with DNA replication. Exp. Cell Res. 163: 287-293, 1986. 2. Moldovan, G.-L.; Pfander, B.; Jentsch, S. : PCNA controls establishment of sister chromatid cohesion during S phase. Molec. Cell 23: 723-732, 2006. 3. Webb, G.; Parsons, P.; Chenevix-Trench, G. : Localization of the gene for human proliferating nuclear antigen/cyclin by in situ hybridization. Hum. Genet. 86: 84-86, 1990.

Uniprot ID: P04961

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG2a

Phospho_site:

Clone Number: Clone: PC 10

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight: 28749 MW

Gene ID: Human(5111), Mouse(18538), Rat(25737)

Protein Name: Proliferating cell nuclear antigen

Gene Full Name: proliferating cell nuclear antigen

Synonyms: Cyclin; DNA POLYMERASE DELTA AUXILIARY PROTEIN