Anti-PCBD1 Antibody Picoband®

SKU: A07264-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: WB,ICC,IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PCBD1

Specificity:

Background: Pterin-4-alpha-carbinolamine dehydratase is an enzyme that in humans is encoded by the PCBD1 gene. This gene encodes a member of the pterin-4-alpha-carbinolamine dehydratase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein functions as both a dehydratase involved in tetrahydrobiopterin biosynthesis, and as a cofactor for HNF1A-dependent transcription. A deficiency of this enzyme leads to hyperphenylalaninemia. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human PCBD1 recombinant protein (Position: M1-T105). Human PCBD1 shares 99% and 100% amino acid (aa) sequence identity with mouse and rat PCBD1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bayle, J. H., Randazzo, F., Johnen, G., Kaufman, S., Nagy, A., Rossant, J., Crabtree, G. R. Hyperphenylalaninemia and impaired glucose tolerance in mice lacking the bifunctional DCoH gene. J. Biol. Chem. 277: 28884-28891, 2002. 2. Citron, B. A., Kaufman, S., Milstien, S., Naylor, E. W., Greene, C. L., Davis, M. D. Mutation in the 4a-carbinolamine dehydratase gene leads to mild hyperphenylalaninemia with defective cofactor metabolism. Am. J. Hum. Genet. 53: 768-774, 1993. 3. Ferre, S., de Baaij, J. H. F., Ferreira, P., Germann, R., de Klerk, J. B. C., Lavrijsen, M., van Zeeland, F., Venselaar, H., Kluijtmans, L. A. J., Hoenderop, J. G. J., Bindels, R. J. M. Mutations in PCBD1 cause hypomagnesemia and renal magnesium wasting. J. Am. Soc. Nephrol. 25: 574-586, 2014.

Uniprot ID: P61457

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 12 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 5092

Protein Name: Pterin-4-alpha-carbinolamine dehydratase

Gene Full Name: pterin-4 alpha-carbinolamine dehydratase 1

Synonyms: DCoH; PCBD; PCBD1; PCD; PHS