Anti-PACSIN1 Antibody Picoband®

SKU: A06188-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PACSIN1

Specificity:

Background: Protein kinase C and casein kinase substrate in neurons protein 1 is an enzyme that in humans is encoded by the PACSIN1 gene. PACSIN1 was originally identified as a molecular link between membrane trafficking and actin organization. PACSIN1 interacts with vesicle-associated proteins and plays a role in endocytosis and endosomal recycling. In addition, it has a role in actin remodeling and microtubule nucleation.

Immunogen: E.coli-derived human PACSIN1 recombinant protein (Position: Q76-E359). Human PACSIN1 shares 95.8% and 96.5% amino acid (aa) sequence identity with mouse and rat PACSIN1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Liu, Y., Lv, K., Li, Z., Yu, A. C. H., Chen, J., Teng, J. PACSIN1, a Tau-interacting protein, regulates axonal elongation and branching by facilitating microtubule instability. J. Biol. Chem. 287: 39911-39924, 2012 2. Modregger, J., DiProspero, N.A., Charles, V., Tagle, D.A., Plomann, M. PACSIN 1 interacts with huntingtin and is absent from synaptic varicosities in presymptomatic Huntington's disease brains. Hum. Molec. Genet. 11: 2547-2558, 2002. 3. Nagase, T., Kikuno, R., Ishikawa, K., Hirosawa, M., Ohara, O. Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 65-73, 2000.

Uniprot ID: Q9BY11

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 51 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 29993

Protein Name: Protein kinase C and casein kinase substrate in neurons protein 1

Gene Full Name: protein kinase C and casein kinase substrate in neurons 1

Synonyms: KIAA1379, PACSIN1, SDPI