Anti-OS9 Antibody Picoband®

SKU: A04158-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: OS9

Specificity:

Background: Protein OS-9 is a protein that in humans is encoded by the OS9 gene. This gene encodes a protein that is highly expressed in osteosarcomas. This protein binds to the hypoxia-inducible factor 1 (HIF-1), a key regulator of the hypoxic response and angiogenesis, and promotes the degradation of one of its subunits. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Immunogen: E.coli-derived human OS9 recombinant protein (Position: Q56-E635).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Baek, J. H., Mahon, P. C., Oh, J., Kelly, B., Krishnamachary, B., Pearson, M., Chan, D. A., Giaccia, A. J., Semenza, G. L. OS-9 interacts with hypoxia-inducible factor 1-alpha and prolyl hydroxylases to promote oxygen-dependent degradation of HIF-1-alpha. Molec. Cell 17: 503-512, 2005. 2. Bhamidipati, A., Denic, V., Quan, E. M., Weissman, J. S. Exploration of the topological requirements of ERAD identifies Yos9p as a lectin sensor of misfolded glycoproteins in the ER lumen. Molec. Cell 19: 741-751, 2005. 3. Friedmann, E., Salzberg, Y., Weinberger, A., Shaltiel, S., Gerst, J. E. YOS9, the putative yeast homolog of a gene amplified in osteosarcomas, is involved in the endoplasmic reticulum (ER)-Golgi transport of GPI-anchored proteins. J. Biol. Chem. 277: 35274-35281, 2002.

Uniprot ID: Q13438

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 83 kDa

Calculated Molecular Weight: 90314 MW

Gene ID: 10956

Protein Name: Protein OS-9

Gene Full Name: OS9 endoplasmic reticulum lectin

Synonyms: Amplified in osteosarcoma 9; OS 9; OS9; Protein OS 9