Anti-NUF2 Antibody Picoband®

SKU: A03788-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NUF2

Specificity:

Background: Kinetochore protein Nuf2 is a protein that in humans is encoded by the NUF2 gene. This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.

Immunogen: E.coli-derived human NUF2 recombinant protein (Position: M1-Q297).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. DeLuca, J. G., Moree, B., Hickey, J. M., Kilmartin, J. V., Salmon, E. D. hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells. J. Cell Biol. 159: 549-555, 2002. 2. Liu, D., Ding, X., Du, J., Cai, X., Huang, Y., Ward, T., Shaw, A., Yang, Y., Hu, R., Jin, C., Yao, X. Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment. J. Biol. Chem. 282: 21415-21424, 2007. 3. Nabetani, A., Koujin, T., Tsutsumi, C., Haraguchi, T., Hiraoka, Y. A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint. Chromosoma 110: 322-334, 2001.

Uniprot ID: Q9BZD4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 54 kDa

Calculated Molecular Weight: 64133 MW

Gene ID: 83540

Protein Name: Kinetochore protein Nuf2

Gene Full Name: NUF2 component of NDC80 kinetochore complex

Synonyms: CDCA1; hNuf2; hNuf2R; hsNuf2; Kinetochore protein Nuf2; NUF2; NUF2R