Anti-NT5C Antibody Picoband®

SKU: A10650-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NT5C

Specificity:

Background: 5', 3'-nucleotidase, cytosolic, also known as 5'(3')-deoxyribonucleotidase, cytosolic type (cdN) or deoxy-5'-nucleotidase 1 (dNT-1), is an enzyme that in humans is encoded by the NT5C gene on chromosome 17. This gene encodes a nucleotidase that catalyzes the dephosphorylation of the 5' deoxyribonucleotides (dNTP) and 2'(3')-dNTP and ribonucleotides, but not 5' ribonucleotides. Of the different forms of nucleotidases characterized, this enzyme is unique in its preference for 5'-dNTP. It may be one of the enzymes involved in regulating the size of dNTP pools in cells. Alternatively spliced transcript variants have been found for this gene.

Immunogen: E.coli-derived human NT5C recombinant protein (Position: H35-R179).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Anderson, J. A., Teng, Y.-S., Giblett, E. R. Stains for six enzymes potentially applicable to chromosomal assignment by cell hybridization. Cytogenet. Cell Genet. 14: 295-299, 1975. 2. Hoglund, L., Reichard, P. Cytoplasmic 5-prime(3-prime)-nucleotidase from human placenta. J. Biol. Chem. 265: 6589-6595, 1990. 3. Paglia, D. E., Valentine, W. N., Brockway, R. A. Identification of thymidine nucleotidase and deoxyribonucleotidase activities among normal isozymes of 5-prime-nucleotidase in human erythrocytes. Proc. Nat. Acad. Sci. 81: 588-592, 1984.

Uniprot ID: Q8TCD5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 24 kDa

Calculated Molecular Weight: 29370 MW

Gene ID: 30833

Protein Name: 5'

Gene Full Name: 5', 3'-nucleotidase, cytosolic

Synonyms: Deoxy 5 nucleotidase 1, DNT, dNT 1, DNT1, NT5C, P5N2, PN I, PN II, UMPH2