Anti-NRCAM Antibody Picoband®

SKU: A03746-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/ml

Gene Name: NRCAM

Specificity:

Background: Enables protein binding activity involved in heterotypic cell-cell adhesion. Involved in cell-cell adhesion; nervous system development; and regulation of postsynapse organization. Acts upstream of or within several processes, including neuron development; neuronal action potential propagation; and regulation of neuron projection development. Located in axon; plasma membrane; and synapse. Is active in glutamatergic synapse and postsynaptic density membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; integumental system; and sensory organ. Used to study autism spectrum disorder. Orthologous to human NRCAM (neuronal cell adhesion molecule).

Immunogen: E.coli-derived human NRCAM recombinant protein (Position: R207-Q1025). Mouse NRCAM shares 91.7% and 97.1% amino acid (aa) sequence identity with human and rat NRCAM, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Custer, A. W., Kazarinova-Noyes, K., Sakurai, T., Xu, X., Simon, W., Grumet, M., Shrager, P. The role of the ankyrin-binding protein NrCAM in node of Ranvier formation. J. Neurosci. 23: 10032-10039, 2003. 2. Demyanenko, G. P., Mohan, V., Zhang, X., Brennaman, L. H., Dharbal, K. E. S., Tran, T. S., Manis, P. B., Maness, P. F. Neural cell adhesion molecule NrCAM regulates semaphorin 3F-induced dendritic spine remodeling. J. Neurosci. 34: 11274-11287, 2014. 3. Dry, K., Kenwrick, S., Rosenthal, A., Platzer, M. The complete sequence of the human locus for NgCAM-related cell adhesion molecule reveals a novel alternative exon in chick and man and conserved genomic organization for the L1 subfamily. Gene 273: 115-122, 2001.

Uniprot ID: Q810U4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 136 kDa

Calculated Molecular Weight: 144 kDa

Gene ID: 319504

Protein Name: Neuronal cell adhesion molecule

Gene Full Name: neuronal cell adhesion molecule

Synonyms: Ng-CAM-related; Ng CAM related; Neuronal surface protein Bravo; neuronal cell adhesion molecule; KIAA0343