Anti-NOP2 Antibody Picoband®

SKU: A04830-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NOP2

Specificity:

Background: Enables RNA binding activity. Involved in positive regulation of cell population proliferation; regulation of signal transduction by p53 class mediator; and ribosomal large subunit assembly. Located in nucleolus.

Immunogen: E.coli-derived human NOP2 recombinant protein (Position: L210-Q747).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Baens, M., Chaffanet, M., Aerssens, J., Cassiman, J.-J., Marynen, P. Assignment of the gene for the human proliferating cell nucleolar protein P120 (NOL1) to chromosome 12p13 by fluorescence in situ hybridization and polymerase chain reaction with somatic cell hybrids. Genomics 21: 296-297, 1994. 2. Fonagy, A., Swiderski, C., Wilson, A., Bolton, W., Kenyon, N., Freeman, J. W. Cell cycle regulated expression of nucleolar antigen P120 in normal and transformed human fibroblasts. J. Cell. Physiol. 154: 16-27, 1993. 3. Freeman, J. W., Busch, R. K., Gyorkey, F., Gyorkey, P., Ross, B. E., Busch, H. Identification and characterization of a human proliferation-associated nucleolar antigen with a molecular weight of 120,000 expressed in early G1 phase. Cancer Res. 48: 1244-1251, 1988.

Uniprot ID: P46087

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 120 kDa

Calculated Molecular Weight:

Gene ID: 4839

Protein Name: 28S rRNA

Gene Full Name: NOP2 nucleolar protein

Synonyms: NOL1; NOP120; NOP2; NSUN1; Nucleolar protein 1; Nucleolar protein 2 homolog; p120