Anti-NLRP6 Antibody Picoband®

SKU: A03636-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: NLRP6

Specificity:

Background: The protein encoded by this gene binds arginine-vasopressin and may be involved in the arginine-vasopressin-mediated regulation of renal salt-water balance. The encoded protein also mediates inflammatory responses in the colon to allow recovery from intestinal epithelial damage and protects against tumorigenesis and the development of colitis. Finally, this protein can increase activation of NF-kappa-B, activation of CASP1 through interaction with ASC, and cAMP accumulation. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human NLRP6 recombinant protein (Position: R14-E887). Human NLRP6 shares 71.7% and 72.9% amino acid (aa) sequence identity with mouse and rat NLRP6, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Anand, P. K., Malireddi, R. K. S., Lukens, J. R., Vogel, P., Bertin, J., Lamkanfi, M., Kanneganti, T.-D. NLRP6 negatively regulates innate immunity and host defence against bacterial pathogens. Nature 488: 389-393, 2012. 2. Elinav, E., Strowig, T., Kau, A. L., Henao-Mejia, J., Thaiss, C. A., Booth, C. J., Peaper, D. R., Bertin, J., Eisenbarth, S. C., Gordon, J. I., Flavell, R. A. NLRP6 inflammasome regulates colonic microbial ecology and risk for colitis. Cell 145: 745-757, 2011. 3. Grenier, J. M., Wang, L., Manji, G. A., Huang, W.-J., Al-Garawi, A., Kelly, R., Carlson, A., Merriam, S., Lora, J. M., Briskin, M., DiStefano, P. S., Bertin, J. Functional screening of five PYPAF family members identifies PYPAF5 as a novel regulator of NF-kappa-B and caspase-1. FEBS Lett. 530: 73-78, 2002.

Uniprot ID: P59044

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 105 kDa

Calculated Molecular Weight: 99 kDa

Gene ID: 171389

Protein Name: NACHT, LRR and PYD domains-containing protein 6

Gene Full Name: NLR family pyrin domain containing 6

Synonyms: NLRP6; NALP6; PYPAF5; NACHT, LRR and PYD domains-containing protein 6; Angiotensin II/vasopressin receptor; PYRIN-containing APAF1-like protein 5