Anti-NIRF Antibody Picoband® (monoclonal, 6B5)

SKU: M06294-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Rat

Gene Name: UHRF2

Specificity:

Background: E3 ubiquitin-protein ligase UHRF2 is an enzyme that in humans is encoded by the UHRF2 gene. This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis. The encoded protein contains an NIRF_N domain, a PHD finger, a set- and ring-associated (SRA) domain, and a RING finger domain and several of these domains have been shown to be essential for the regulation of cell proliferation. This protein may also have a role in intranuclear degradation of polyglutamine aggregates. Alternative splicing results in multiple transcript variants some of which are non-protein coding.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human NIRF, identical to the related mouse and rat sequences.

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Entrez Gene: UHRF2 ubiquitin-like, containing PHD and RING finger domains, 2. 2. Mori T, Li Y, Hata H, Ono K, Kochi H (Aug 2002). NIRF, a novel RING finger protein, is involved in cell-cycle regulation. Biochem Biophys Res Commun 296 (3): 530–6.

Uniprot ID: Q96PU4

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 6B5

Observed Molecular Weight: 90 kDa

Calculated Molecular Weight:

Gene ID: 115426

Protein Name: E3 ubiquitin-protein ligase UHRF2

Gene Full Name: ubiquitin like with PHD and ring finger domains 2

Synonyms: ubiquitin like with PHD and ring finger domains 2; NIRF; RNF107; TDRD23; URF2; UHRF2