Anti-NeuN/Rbfox3 Antibody Picoband®

SKU: A11954-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: ELISA,IF,IHC,WB

Application Details: Western blot, 0.1-0.25 μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunofluorescence, 5 μg/ml, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Rbfox3

Specificity:

Background: RNA binding protein, fox-1 homolog (C. elegans) 3 (Rbfox3) is a protein that in humans is encoded by the RBFOX3 gene. This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Immunogen: E.coli-derived mouse NeuN/Rbfox3 recombinant protein (Position: H28-D304).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Dredge, B. K., Jensen, K. B. NeuN/Rbfox3 nuclear and cytoplasmic isoforms differentially regulate alternative splicing and nonsense-mediated decay of Rbfox2. PLoS One 6: e21585, 2011. Note: Electronic Article. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 6/22/2016. 3. Kim, K. K., Adelstein, R. S., Kawamoto, S. Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors. J. Biol. Chem. 284: 31052-31061, 2009.

Uniprot ID: Q8BIF2

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 46-55 kDa

Calculated Molecular Weight:

Gene ID: 52897

Protein Name: RNA binding protein fox-1 homolog 3

Gene Full Name: RNA binding protein, fox-1 homolog (C. elegans) 3

Synonyms: Rbfox3; D11Bwg0517e; Hrnbp3; RNA binding protein fox-1 homolog 3; Fox-1 homolog C; Hexaribonucleotide-binding protein 3; Fox-3; Neuronal nuclei antigen; NeuN antigen