Anti-NEK9 Antibody Picoband®

SKU: A04660-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: NEK9

Specificity:

Background: This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein is activated in mitosis and, in turn, activates other family members during mitosis. This protein also mediates cellular processes that are essential for interphase progression.

Immunogen: E.coli-derived human NEK9 recombinant protein (Position: K81-K945). Human NEK9 shares 94.8% amino acid (aa) sequence identity with mouse NEK9.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Belham, C., Roig, J., Caldwell, J. A., Aoyama, Y., Kemp, B. E., Comb, M., Avruch, J. A mitotic cascade of NIMA family kinases: Nercc1/Nek9 activates the Nek6 and Nek7 kinases. J. Biol. Chem. 278: 34897-34909, 2003. 2. Casey, J. P., Brennan, K., Scheidel, N., McGettigan, P., Lavin, P. T., Carter, S., Ennis, S., Dorkins, H., Ghali, N., Blacque, O. E., McGee, M. M., Murphy, H., Lynch, S. A. Recessive NEK9 mutation causes a lethal skeletal dysplasia with evidence of cell cycle and ciliary defects. Hum. Molec. Genet. 25: 1824-1835, 2016. 3. Holland, P. M., Milne, A., Garka, K., Johnson, R. S., Willis, C., Sims, J. E., Rauch, C. T., Bird, T. A., Virca, G. D. Purification, cloning, and characterization of Nek8, a novel NIMA-related kinase, and its candidate substrate Bicd2. J. Biol. Chem. 277: 16229-16240, 2002.

Uniprot ID: Q8TD19

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 120 kDa

Calculated Molecular Weight: 107 kDa

Gene ID: 91754

Protein Name: Serine/threonine-protein kinase Nek9

Gene Full Name: NIMA related kinase 9

Synonyms: KIAA1995; Nek8; NEK9; NERCC; NERCC1; Nercc1 kinase; NimA related kinase 8; NimA related protein kinase 9