Anti-N WASP/WASL Antibody Picoband®

SKU: A05438-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: WASL

Specificity:

Background: Neural Wiskott-Aldrich syndrome protein is a protein that in humans is encoded by the WASL gene. This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension.

Immunogen: E.coli-derived human N WASP/WASL recombinant protein (Position: Q5-H211).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Cotta-de-Almeida, V., Westerberg, L., Maillard, M. H., Onaldi, D., Wachtel, H., Meelu, P., Chung, U., Xavier, R., Alt, F. W., Snapper, S. B. Wiskott-Aldrich syndrome protein (WASP) and N-WASP are critical for T cell development. Proc. Nat. Acad. Sci. 104: 15424-15429, 2007. 2. Fukuoka, M., Miki, H., Takenawa, T. Identification of N-WASP homologs in human and rat brain. Gene 196: 43-48, 1997. 3. Guenin-Mace, L., Veyron-Churlet, R., Thoulouze, M.-I., Romet-Lemonne, G., Hong, H., Leadlay, P. F., Danckaert, A., Ruf, M.-T., Mostowy, S., Zurzolo, C., Bousso, P., Chretien, F., Carlier, M.-F., Demangel, C. Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation. J. Clin. Invest. 123: 1501-1512, 2013.

Uniprot ID: O00401

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 70 kDa

Calculated Molecular Weight: 39411 MW

Gene ID: 8976

Protein Name: Actin nucleation-promoting factor WASL

Gene Full Name: WASP like actin nucleation promoting factor

Synonyms: N WASP; NWASP; WASL; Wiskott Aldrich syndrome like