Anti-MYO1G Antibody Picoband®

SKU: A08448-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MYO1G

Specificity:

Background: Myosin IG, also known as myosin 1G and MYO1G, is a protein that in humans is encoded by the MYO1G gene. MYO1G is a plasma membrane-associated class I myosin (see MIM 601478) that is abundant in T and B lymphocytes and mast cells.

Immunogen: E.coli-derived human MYO1G recombinant protein (Position: H252-Q966). Human MYO1G shares 90.2% amino acid (aa) sequence identity with mouse MYO1G.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. de Bueger, M., Bakker, A., van Rood, J. J., van der Woude, F., Goulmy, E. Tissue distribution of human minor histocompatibility antigens: ubiquitous versus restricted tissue distribution indicates heterogeneity among human cytotoxic T lymphocyte-defined non-MHC antigens. J. Immun. 149: 1788-1794, 1992. 2. den Haan, J. M. M., Sherman, N. E., Blokland, E., Huczko, E., Koning, F., Drijfhout, J. W., Skipper, J., Shabanowitz, J., Hunt, D. F., Engelhard, V. H., Goulmy, E. Identification of a graft versus host disease-associated human minor histocompatibility antigen. Science 268: 1476-1480, 1995. 3. Maravillas-Montero, J. L., Lopez-Ortega, O., Patino-Lopez, G., Santos-Argumedo, L. Myosin 1g regulates cytoskeleton plasticity, cell migration, exocytosis, and endocytosis in B lymphocytes. Europ. J. Immun. 44: 877-886, 2014.

Uniprot ID: B0I1T2

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 116 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 64005

Protein Name: Unconventional myosin-Ig [Cleaved into: Minor histocompatibility antigen HA-2

Gene Full Name: myosin IG

Synonyms: Myosin 1G; Unconventional myosin-Ig; Minor histocompatibility antigen HA-2; mHag HA-2; MYO1G