Anti-MYO1E Antibody Picoband®

SKU: A04139-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: MYO1E

Specificity:

Background: This gene encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. Class I myosins are characterized by a head (motor) domain, a regulatory domain and a either a short or long tail domain. Among the class I myosins, this protein is distinguished by a long tail domain that is involved in crosslinking actin filaments. This protein localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in this gene are the cause of focal segmental glomerulosclerosis-6. This gene has been referred to as myosin IC in the literature but is distinct from the myosin IC gene located on chromosome 17.

Immunogen: E.coli-derived human MYO1E recombinant protein (Position: A221-Q1065). Human MYO1E shares 95.9% and 95.3% amino acid (aa) sequence identity with mouse and rat MYO1E, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bement, W. M., Wirth, J. A., Mooseker, M. S. Cloning and mRNA expression of human unconventional myosin-IC: a homologue of amoeboid myosins-I with a single IQ motif and an SH3 domain. J. Molec. Biol. 243: 356-363, 1994. 2. Hasson, T., Skowron, J. F., Gilbert, D. J., Avraham, K. B., Perry, W. L., Bement, W. M., Anderson, B. L., Sherr, E. H., Chen, Z.-Y., Greene, L. A., Ward, D. C., Corey, D. P., Mooseker, M. S., Copeland, N. G., Jenkins, N. A. Mapping of unconventional myosins in mouse and human. Genomics 36: 431-439, 1996. 3. Krendel, M., Kim, S. V., Willinger, T., Wang, T., Kashgarian, M., Flavell, R. A., Mooseker, M. S. Disruption of myosin 1e promotes podocyte injury. J. Am. Soc. Nephrol. 20: 86-94, 2009.

Uniprot ID: Q12965

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 127 kDa

Calculated Molecular Weight: 127 kDa

Gene ID: 4643

Protein Name: Unconventional myosin-Ie

Gene Full Name: myosin IE

Synonyms: HuncM IC; MYO1E; Myosin Ic; myosin IE