Anti-Myelin Basic Protein/MBP Antibody Picoband®

SKU: PA1050

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat,Pig

Applications: IF,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, Pig
Immunofluorescence, 5μg/ml, Mouse, Rat

Gene Name: MBP

Specificity: No cross reactivity with other proteins.

Background: Myelin basic protein (MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein, identical to the related rat and mouse sequences.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Marty MC, Alliot F, Rutin J, Fritz R, Trisler D, Pessac B.The myelin basic protein gene is expressed in differentiated blood cell lineages and in hemopoietic progenitors. Proc Natl Acad Sci U S A. 2002 Jun 25; 99(13):8856-61. 2. Streicher R, Stoffel W.The organization of the human myelin basic protein gene. Comparison with the mouse gene. Biol Chem Hoppe Seyler. 1989 May; 370(5):503-10. 3. McLaurin JA, Hafler DA, Antel JP.Reactivity of normal T-cell lines to MBP isolated from normal and multiple sclerosis white matter. J Neurol Sci. 1995 Feb; 128(2):205-11. 4. Iester A, Vignola S, Callegarini L, Gimelli G, Alpigiani MG. [18q syndrome with deficiency of myelin basic protein (MBP)] Pediatr Med Chir. 1996 Mar-Apr; 18(2):201-5. 5. Tranquill LR, Skinner E, Campagnoni C, Vergelli M, Hemmer B, Muraro P, Martin R, McFarland HF, Campagnoni AT, Voskuhl RR. Human T lymphocytes specific for the immunodominant 83-99 epitope of myelin basic protein: recognition of golli MBP HOG 7. J Neurosci Res. 1996 Sep 15; 45(6):820-8.

Uniprot ID: P02686

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 17-33 kDa

Calculated Molecular Weight: 33117 MW

Gene ID: 4155

Protein Name: Platelet glycoprotein 4

Gene Full Name: myelin basic protein

Synonyms: MBP; Myelin A1 protein; Myelin basic protein