Anti-MIEF1 Antibody Picoband®

SKU: A06887-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: MIEF1

Specificity:

Background: Mitochondrial dynamic protein MID51 (MID51) also known as mitochondrial elongation factor 1 (MIEF1) or Smith-Magenis syndrome chromosome region candidate gene 7 protein-like (SMCR7L) is a protein that in humans is encoded by the SMCR7L gene. The SMCR7L gene codes for a protein that has been called MiD51/MIEF1 and shown to regulate mitochondrial fission by interacting with the proteins Drp1 and FIS1.

Immunogen: E.coli-derived human MIEF1 recombinant protein (Position: S189-T463).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Hartz, P. A. Personal Communication. Baltimore, Md. 10/25/2013. 2. Palmer, C. S., Elgass, K. D., Parton, R. G., Osellame, L. D., Stojanovski, D., Ryan, M. T. Adaptor proteins MiD49 and MiD51 can act independently of Mff and Fis1 in Drp1 recruitment and are specific for mitochondrial fission. J. Biol. Chem. 288: 27584-27593, 2013. 3. Palmer, C. S., Osellame, L. D., Laine, D., Koutsopoulos, O. S., Frazier, A. E., Ryan, M. T. MiD49 and MiD51, new components of the mitochondrial fission machinery. EMBO Rep. 12: 565-573, 2011.

Uniprot ID: Q9NQG6

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 51 kDa

Calculated Molecular Weight: 51 kDa

Gene ID: 54471

Protein Name: Mitochondrial dynamics protein MIEF1

Gene Full Name: mitochondrial elongation factor 1

Synonyms: HSU79252; MID51; MIEF1; SMCR7L