Anti-METAP2 Antibody Picoband®

SKU: A03648-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat,Monkey

Applications: WB,IHC,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: METAP2

Specificity:

Background: Methionine aminopeptidase 2 is an enzyme that in humans is encoded by the METAP2 gene. The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human METAP2 recombinant protein (Position: E201-R474).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Datta, B., Chakrabarti, D., Roy, A. L., Gupta, N. K. Roles of a 67-kDa polypeptide in reversal of protein synthesis inhibition in heme-deficient reticulocyte lysate. Proc. Nat. Acad. Sci. 85: 3324-3328, 1988. 2. Griffith, E. C., Su, Z., Niwayama, S., Ramsay, C. A., Chang, Y- H., Liu, J. O. Molecular recognition of angiogenesis inhibitors fumagillin and ovalicin by methionine aminopeptidase 2. Proc. Nat. Acad. Sci. 95: 15183-15188, 1998. 3. Gross, M. B. Personal Communication. Baltimore, Md. 5/21/2014.

Uniprot ID: P50579

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 67 kDa

Calculated Molecular Weight: 28461 MW

Gene ID: 10988

Protein Name: Methionine aminopeptidase 2

Gene Full Name: methionyl aminopeptidase 2

Synonyms: MAP 2; MAP2; MetAP 2; METAP2; Methionine aminopeptidase 2; methionyl aminopeptidase 2; MNPEP; p67; p67eIF2; Peptidase M 2