Anti-Mast Cell Chymase/CMA1 Antibody Picoband®

SKU: PB9055

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human

Gene Name: CMA1

Specificity: No cross reactivity with other proteins.

Background: Chymase is a major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. Chymase is mapped to 14q12. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II.

Immunogen: E.coli-derived human CMA1 recombinant protein (Position: I22-N247). Human CMA1 shares 75% and 74% amino acid (aa) sequences identity with mouse and rat CMA1, respectively.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Caughey, GH. Mast cell tryptases and chymases in inflammation and host defense. Immu Revs 2007 (217): 141-154. 2. Chmelar, J., Oliveira, C. J., Rezacova, P., Francischetti, I. M. B., Kovarova, Z., Pejler, G., Kopacek, P., Ribeiro, J. M. C., Mares, M., Kopecky, J., Kotsyfakis, M. A tick salivary protein targets cathepsin G and Chymase and inhibits host inflammation and platelet aggregation. Blood 117: 736-744, 2011. 3. Urata, H., Boehm, K. D., Philip, A., Kinoshita, A., Gabrovsek, J., Bumpus, F. M., Husain, A.Cellular localization and regional distribution of an angiotensin II-forming Chymase in the heart. J. Clin. Invest. 91: 1269-1281, 1993.

Uniprot ID: P23946

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight: 27325 MW

Gene ID: 1215

Protein Name: Desmin

Gene Full Name: chymase 1

Synonyms: Alpha chymase; chymase; chymase 1; mast cell; CMA1; CYH; CYM; Mast Cell Chymase; Mast cell protease I; Mcpt 5; Mcpt5; MCT1