Anti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody Picoband®

SKU: PB9064

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Rat

Applications: IHC,WB

Application Details: Western blot, 0.1-0.5 μg/ml, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Rat

Gene Name: CCL4

Specificity: No cross reactivity with other proteins.

Background: Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in human is encoded by the CCL4 gene. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize CCL4. Based on functional analysis of CCL4, one or more additional beta chemokine receptors that transduce CCL4 signals must also exist. MIP-1alpha and CCL4 have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines (11, 14).

Immunogen: E.coli-derived rat CCL4 recombinant protein (Position: A24-N92). Rat CCL4 shares 80% and 86% amino acid (aa) sequences identity with human and mouse CCL4, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Irving SG, Zipfel PF, Balke J, McBride OW, Morton CC, Burd PR, Siebenlist U, Kelly K (June 1990). Nucleic Acids Res. 18 (11): 3261–70. 2. Bystry RS, Aluvihare V, Welch KA, Kallikourdis M, Betz AG (December 2001). B cells and professional APCs recruit regulatory T cells via CCL4. Nat. Immunol. 2 (12): 1126–32. 3. Cocchi F, DeVico AL, Garzino-Demo A, Arya SK, Gallo RC, Lusso P (December 1995). Science (journal) 270 (5243): 1811–5. 4. Kamin-Lewis R, Abdelwahab SF, Trang C, Baker A, DeVico AL, Gallo RC, Lewis GK (July 2001).

Uniprot ID: P50230

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 36 kDa

Calculated Molecular Weight: 10234 MW

Gene ID: 116637

Protein Name: C-C motif chemokine 4

Gene Full Name: C-C motif chemokine ligand 4

Synonyms: ACT 2; ACT2; CCL4; MIP 1 beta; HC21; LAG-1; MIP1B; SCYA4; G-26; LAG1; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta