Anti-LYPLA1 Antibody Picoband®

SKU: A07249-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: LYPLA1

Specificity:

Background: Acyl-protein thioesterase 1 is an enzyme that in humans is encoded by the LYPLA1 gene. This gene encodes a member of the alpha/beta hydrolase superfamily. The encoded protein functions as a homodimer, exhibiting both depalmitoylating as well as lysophospholipase activity, and may be involved in Ras localization and signaling. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 4, 6, and 7.

Immunogen: E.coli-derived human BCL3 recombinant protein (Position: E118-A366). Human BCL3 shares 86.7% amino acid (aa) sequence identity with mouse BCL3.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Milde, S., Coleman, M. P. Identification of palmitoyltransferase and thioesterase enzymes that control the subcellular localization of axon survival factor nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2). J. Biol. Chem. 289: 32858-32870, 2014. 2. Wang, A., Yang, H.-C., Friedman, P., Johnson, C. A., Dennis, E. A. A specific human lysophospholipase: cDNA cloning, tissue distribution and kinetic characterization. Biochim. Biophys. Acta 1437: 157-169, 1999.

Uniprot ID: O75608

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 25 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 10434

Protein Name: Acyl-protein thioesterase 1

Gene Full Name: lysophospholipase 1

Synonyms: Acyl protein thioesterase 1; APT 1; APT1; hAPT1; LPL I; LPL1; LYPLA1; Lysophospholipase 1; lysophospholipase I; LYSOPLA; LysoPLA I